ADIG诱导牛成肌细胞转分化及相关基因的表达分析

doi:10.11843/j.issn.0366-6964.2016.04.004

Abstract

Abstract:

The aim of this study was to provide evidence about the role of ADIG gene in bovine adipose tissue during growth and development，and to lay a theoretical basis for further study of the molecular mechanisms of bovine fat metabolism and meat quality improvement through inducing bovine myoblasts trans-differentiating into fat with overexpressing ADIG gene in bovine myoblasts.We induced bovine myoblasts trans-differentiating with AD-ADIG recombinant adenovirus，observed the lipid droplets after Oil Red O staining，collected cells to extract RNA at the 0^th，2^nd，4^th，6^th，8^th，10^th，12^th，14^th day，respectively，and detected relative expressions of fat-related genes ADIG，PPARγ，C/EBPα，SREBPF1，FABP4，FAS as well as the muscle-related gene MyoD by real-time quantitative PCR.The real-time quantitative PCR results showed that during the induced trans-differentiation process，ADIG gene was expressed at high levels at the 2^nd，4^th，6^th，8^th，10^th，12^th，14^th day，and lipid droplets increased significantly.The relative expression of the fat-related genes PPARγ，SREBPF1 and FAS at late stages were obviously higher in AD-ADIG group than that in other groups，and the relative expression of muscle-related gene MyoD decreased at early stages，and was obviously lower in AD-ADIG group than that in other groups.The result indicate that ADIG gene play important roles in bovine myoblasts induced into fat and bovine myoblast has the potential of differentiating to adipocyte according to the results of Oil Red O staining and related gene relative expression.

CLC Number:

S823
S813.3

MEI Chu-gang，ZHANG Qiong，FU Chang-zhen，LIU Yang，JIANG Bi-jie，CHENG Gong，ZAN Lin-sen. ADIG Inducing the Trans-differentiation of Bovine Myoblasts and Its Related Gene Expression[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(4): 661-670.

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References

［1］孔保华，陶菲，刁新平.中国肉牛产业的现状和发展趋势［J］.肉类研究，2002(1)：10-13，21.
KONG B H，TAO F，DIAO X P.Present status and trend of beef cattle breeding in China［J］.Meat Research，2002(1)：10-13，21.(in Chinese)
［2］KIM J Y，TILLISON K，SMAS C M.Cloning，expression，and differentiation-dependent regulation of SMAF1 in adipogenesis［J］. Biochem Biophys Res Commun，2005，326(1)：36-44.
［3］HE T C，ZHOU S，DA COSTA L T，et al.A simplified system for generating recombinant adenoviruses［J］.Proc Natl Acad Sci U S A，1998，95(5)：2509-2514.
［4］LODDE B M，DELPORTE C，GOLDSMITH C M，et al.A recombinant adenoviral vector encoding functional vasoactive intestinal peptide［J］.Biochem Biophys Res Commun，2004，319(1)：189-192.
［5］WU Z，XIE Y，BUCHER N L，et al.Conditional ectopic expression of C/EBPβ in NIH-3T3 cells induces PPARγ and stimulates adipogenesis［J］.Genes Dev，1995，9(19)：2350-2363.
［6］HONG Y H，OGIHARA Y，HISHIKAWA D，et al.Molecular cloning and expression analysis of cDNA encoding bovine adipogenin［J］.Anim Sci J，2006，77：613-619.
［7］BELFIORE A，GENUA M，MALAGUARNERA R.PPAR-gamma agonists and their effects on IGF-I receptor signaling：implications for cancer［J］.PPAR Res，2009，2009：830501.
［8］BERGER J，MOLLER D E.The mechanisms of action of PPARs［J］.Annu Rev Med，2002，53：409-435.
［9］FEIGE J N，GELMAN L，MICHALIK L，et al.From molecular action to physiological outputs：peroxisome proliferator-activated receptors are nuclear receptors at the crossroads of key cellular functions［J］.Prog Lipid Res， 2006，45(2)：120-159.
［10］LI Y，QI Y，HUANG T H，et al.Pomegranate flower：a unique traditional antidiabetic medicine with dual PPAR-alpha/-gamma activator properties［J］.Diabetes Obes Metab，2008，10(1)：10-17.
［11］LIN F T，LANE M D.CCAAT/enhancer binding protein alpha is sufficient to initiate the 3T3-L1 adipocyte differentiation program［J］.Proc Natl Acad Sci U S A，1994，91(19)：8757-8761.
［12］LINHART H G，ISHIMURA-OKA K，DEMAYO F，et al.C/EBPalpha is required for differentiation of white，but not brown，adipose tissue［J］.Proc Natl Acad Sci U S A，2001，98(22)：12532-12537.
［13］DARLINGTON G J，ROSS S E，MACDOUGALD O A.The role of C/EBP genes in adipocyte differentiation［J］.J Biol Chem，1998，273(46)：30057-30060.
［14］HWANG C S，LOFTUS T M，MANDRUP S，et al.Adipocyte differentiation and leptin expression［J］.Annu Rev Cell Dev Biol，1997，13：231-259.
［15］ASSAF S，HAZARD D，PITEL F，et al.Cloning of cDNA encoding the nuclear form of chicken sterol response element binding protein-2(SREBP-2)，chromosomal localization，and tissue expression of chicken SREBP-1 and-2 genes［J］.Poult Sci，2003，82(1)：54-61.
［16］卓伟华，王继文，蒋立.固醇调节元件结合蛋白在动物脂肪代谢中的研究进展［J］.中国畜牧杂志，2005，41(2)：39-41.
ZHUO W H，WANG J W，JIANG L.Advance in SREBPs in animal lipid metabolism［J］.Chinese Journal of Animal Science，2005，41(2)：39-41.(in Chinese)
［17］JENSEN-URSTAD A P，SEMENKOVICH C F.Fatty acid synthase and liver triglyceride metabolism：housekeeper or messenger?［J］.Biochim Biophys Acta，2012，1821 (5)：747-753.
［18］SWIERCZYN＇SKI J，SLEDZIN＇SKI T.Metabolic and regulatory function of fatty acid synthase［J］.Postepy Biochem，2012，58(2)：175-185.
［19］BIONAZ M，LOOR J J.Gene networks driving bovine milk fat synthesis during the lactation cycle［J］.BMC Genomics，2008，9：366.
［20］SABOURIN L A，RUDNICKI M A.The molecular regulation of myogenesis［J］.Clin Genet，2000，57(1)：16-25.
［21］岑石强，杨志明.成肌细胞在基因治疗和组织工程中的应用综述［J］.中国修复重建外科杂志，1999，13(3)：173-177.
CEN S Q，YANG Z M.A reciew on the application of myoblast on gene therapy and tissue engineering［J］.Chinese Journal of Reparative and Reconstructive Surgery，1999，13(3)：173-177.(in Chinese)

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ADIG Inducing the Trans-differentiation of Bovine Myoblasts and Its Related Gene Expression

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